WebbSequencing was performed by the University of Delaware Sequencing and Genotyping Center on two lanes of Illumina HiSeq 2500 System using paired-end 150 bp reads. 2 Raw reads were assembled into ... Webb6 dec. 2024 · Hybrids between species or diverged lineages contain fundamentally novel genetic combinations but an impaired meiosis often makes them evolutionary dead ends. Here, we explored to what extent and how an aborted meiosis followed by a return-to-growth (RTG) promotes recombination across a panel of 20 yeast diploid backgrounds …
High % of multi mapping reads (multiple loci) #1491 - Github
Webb25 maj 2012 · One strategy for mapping multi mapping reads from an RNA-seq experiment, was to examine the mapping locations of all reads (single and multi mapping reads) and … Webb29 maj 2015 · I recently mapped 8 RNA-Seq samples using STAR. The samples are paired. The data in log.final.out for each sample look very similar to the sample's partner - with one exception. I have one sample that shows 6.57% reads mapped to multiple loci and 32.38 % of reads unmapped: too short. recaro bankruptcy
Aligning RNA-seq reads with STAR (Complete tutorial)
Webb1 sep. 2015 · by default, STAR only outputs reads that map to <=10 loci, others are considered "mapped to too many loci". You can increase this threshold by increasing - … WebbReads that map to more than 10 loci are counted as mapping to too many loci. You can change --outFilterMultimapNmax to increase the threshold. Make sure to also increase … Webb1 juni 2024 · We explore computational strategies for resolving multi-gene mapped reads, such as those implemented in RSEM and STARsolo and find that they provide a partial … recaro bench seat