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Phis2-p53

Webb使用phis2载体，将启动子序列构建在his3报告基因前面的多克隆位点上，对应的转录因子构建在ad载体上即可。使用y187菌株，它相对于ah109菌株在基因组上少了his的报告基 … Webb5 apr. 2024 · The Y187 strain co-expressing pGADT7-LkARF7 and pHIS2-LkBBM1 and the strain co-expressing pGADT7-LkARF19 and pHIS2-LkBBM1 grew normally on TDO medium containing 75 mM 3-AT, ... The bait vectors pGBKT7-p53 and pGBKT7-LaminC were co-transformed into AH109 with the prey vector pGADT7-LargeT as positive and negative …

Y187-pHis2YeastOne-HybridMediaKit 使用说明书

WebbFRFS-0353-2 For Epcos saw filter P/N B4959 REV 1.00 Electro-Photonics LLC reserves the right to modify drawings, configurations, specifications, and design at any time without … Webbp53-His,pLacZ,pHis，pHisi-1. 1、长期保存的储存菌株以冷冻干燥保藏为主，特殊要求的菌种可用其它方法进行保存，保存期可长达几年至几十年。. 2、半储存菌株以含20%甘油的肉汤或液体石腊封存的半固体琼脂为主，在0℃以下保存，保存期为3－6个月。. 3、应用菌株 … how to sight read piano faster https://australiablastertactical.com

质粒表达载体pCMV-BD载体 pCMV-AD载体 pBD-p53载体 pAD …

WebbPlasmid Phis2 1 Cloning Reporter Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article … Webb6 sep. 2024 · 1）pHis2-bait和AD-prey质粒混合作为实验组，将pHis2-bait和空载AD质粒混合作为自激活组。 将实验组和自激活组分别共转到Y187感受态细胞中。 2）用SD/-Leu/ … WebbProbe 2 and Probe 4 sequences as wells mutant Probe 2M and Probe 4M sequences were cloned to pHIS2.1, which drives the expression of HIS3 . GBFs were cloned to pGADT7. Yeast cells were co-transformed with a combination of the indicated plasmids or empty vector and plated onto nonselective (−Leu/−Trp) and selective ... nouns that are intangible

p53-His,pLacZ,pHis，pHisi-1_MZ菌株_宁波明舟生物科技有限公 …

Category:p53基因 - 百度百科-验证

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Phis2-p53

PtrHAT22, as a higher hierarchy regulator, coordinately regulates ...

Webb7 dec. 2024 · For Y1H assays, sequences including the MBS or ERE elements from the promoter of VvMYBPA1 or VvMYBPA2 were cloned into the pHis2 vector. Mutant MBS or ERE elements were used as a negative control. The ORF of VvMYB14 or VvERF104 was cloned into the pGADT7 vector. WebbpHis2: 质粒类型: 酵母单杂交载体: 高拷贝/低拷贝:--启动子:--克隆方法: 多克隆位点，限制性内切酶: 载体大小: 7207bp: 5' 测序引物及序列:--3' 测序引物及序列:--载体标签: TRP1, HIS3: …

Phis2-p53

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http://baiyk.com.cn/ProDetail.aspx?ProID=56 Webb15 nov. 2024 · To eliminate the false positive results of the TF-centered Y1H assay, the bait plasmid pGADT7-bZIP44 was verified for self-activation. pGADT7-bZIP44 + pHIS2-p53 and pGAD53m + pHIS2-p53 were respectively transformed into the Y187 yeast cells, which were spread on SD/-Trp-Leu medium and incubated at 30 ℃ for 3–5 d.

Webbp53his载体价格1200元，p53his载体质粒图谱(Vector map),载体序列(Sequence)见下文。p53his是酵母单杂交载体的p53his。 Webb20 dec. 2024 · b, The effecter and reporter constructs were co-transformed into the yeast strain Y187. p53His2 + pGADT7-p53, positive control, pGADT7-Rec2 vector that encodes murine p53 fused with GAL4 AD; p53His2 + pGADT7-JrDof3, negative control, pHis2 reporter vector that contains the cis-acting DNA consensus sequence recognized by …

Webb24 jan. 2024 · The p53 fragment was from pGBKT7-p53 used for Y2H. ... The promoter cloned in pHIS2.1 is strong autoactivator and the yeast colonies transformed with this is growing even at 100mM 3-AT. http://www.engineeredprocess.com/images/Honeywell_IFM_P531.P532_IOM.pdf

Webb更多详情咨询 www.bnbiotech.com, 021-61496710,15800960770 下面所有质粒和菌株，均为本公司实验室亲自验证过，可以提供技术支持，载体来源于promega,addgene,clontech,novagen,invitrogen可提供质粒图谱，可提供近万种质粒载体、菌株、基因和细胞株,并可提供实验技术外包服务,如基因克隆、质粒构建、蛋白原核、真 …

how to sight rifle scopeWebb4 okt. 2013 · The interactions of pHIS2-p53 (three tandem copies of the cis-acting DNA consensus sequence in pHIS2, which is recognized by p53) with the tested TFs were used as negative controls. Two MYC ( ThMYC4 and ThMYC6 GenBank number: JN166788 and JN166790) were identified to bind to the E-box motif, of which ThMYC6 that bound with … how to sight red dotWebb2.制备感受态并将pHis2-DNA转化进入感受态细胞； 3.SD/-Trp平板检测转化结果。 二、自激活检测 1.制备不同3-AT浓度梯度的SD/-His/-Trp平板，3-AT的浓度一般设定在50-100mM … how to sight scope on rifleWebbPlasmid Phis2 1 Cloning Reporter Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article … nouns synonym and antonymWebbP0153 Mazda HO2S LF Circuit Malfunction. P0153 Mercedes-Benz O2 Sensor Circuit Slow Response Bank 2 Sensor 1. P0153 Mercury O2 Sensor Circuit Slow Response Bank 2 … nouns that can be adjectivesWebb产品名称. p53-His,pLacZ,pHis，pHisi-1. 商品货号. B80888. 中文名称. 酵母单杂交. 冻干物说明. ·冻干首次活化,干粉要全部用完,不能保留,用0.2-0.5m1的培养液或者无菌水溶解,接种 … how to sight sing for beginners pdfWebbpBIND pACT-MyoD pBIND-Id pG5 luc pCMV-BD pCMV-AD pBD-p53 pBD-NF−κB pAD-SV40T pAD-TRAF pFR-luc pGBKT7 pGADT7 pCL1 pGBKT7-53 pGADT7-T pGBKT7-Lam pACT2 AD Y187 AH109 pSos pMyr pSos MAFB pHis2 pHisSi-1 p53blue pGAG424 pLaczi p53his pGAD53m pBridge Y2H Gold Yeast strain Y187 pIND nouns that rhyme with car