WebUniversity, Japan), the GFP-mCherry-LC3B reporter pro-tein has been widely used as an indicator of autophagy, based on the property of GFP, but not mCherry, fluores-cence to be quenched at the acidic pH of autolysosomes. Defects in autophagy are thus manifested by an increased GFP:mCherry ratio. Taking advantage of this property, we WebLC3 is a commonly used marker for measuring autophagic flux. mRFP-GFP-LC3 functions as an autophagy bionsensor via the same mechanism as Rosella: GFP is quenched in …
含传感器 LC3B、p62 或串联传感器 RFP-GFP-LC3B 的 Premo 自噬 …
Web10 apr. 2024 · Main. Macro-autophagy (hereafter termed ‘autophagy’) encapsulates large intracellular cargos in a double-membrane structure named the autophagosome 1, 2. Upon fusion of the autophagosome with ... WebThe LC3B protein plays a critical role in autophagy. Normally, this protein resides in the cytosol, but following cleavage and lipidation with phosphatidylethanolamine, LC3B … produktionstechnologe/-in
Membrane dynamics of ATG4B and LC3 in autophagosome …
WebmCherry具有优异光稳定性，是最通用红色单体（但融合表达时有较弱的齐聚效应） tdTomato具有mCherry相同的光稳定性，亮度更强，是追踪表达水平的理想选择，但为串联二聚体，可以在融合标签大小不干扰蛋白质功能的情况下使用 mStrawberry是最亮的红色单体，但光稳定性要弱于mCherry DsRed是细胞毒性最小的RFP mApple在蛋白的融合表达 … Web8 jul. 2024 · This article describes a cell-based imaging screening workflow developed to monitor autophagic flux using LC3 as a reporter of autophagic flux (mCherry-EGFP-LC3B) in human chondrocytes. Data acquisition is performed using an automated High Content Imaging Screening System microscope. WebGFP-LC3 单荧光自噬指示体系： 利用 LC3 在自噬形成过程中发生聚集的现象构建了 GFP-LC3 指示体系，无自噬时，GFP-LC3 融合蛋白弥散在胞浆中；自噬形成时，GFP-LC3 融合蛋白转位至自噬体膜，在荧光显微镜下形成多个明亮的绿色荧光斑点，一个斑点相当于一个自噬体，可以通过计数来评价自噬活性的高低。 mRFP-GFP-LC3 双荧光自噬指示体系： … produktions und kostentheorie