WebReagent: DIG Wash and Block Buffer Set (Sigma-Aldrich; code no. 11585762001) Lane 1: 500 ng of total RNA Lane 2: 100 ng of total RNA Lane 3: 20 ng of total RNA Fluorescence in situ hybridization of MALAT1 ncRNA Cells: HeLa Probe: MALAT1 ncRNA (RefSeq ID: NR_002819.3, region 6641-7113) Antibody: Anti-Digoxigenin (DIG) mAb (M227-3), 1 g/mL WebA specially formulated TBS, pH 7.7, designed to provide superior pH stability. This wash buffer works well for manual, capillary gap and automated immunohistochemistry applications such as the intelliPATH FLX as well as other laboratory procedures requiring a high-quality TBS buffer. The surfactant allows for reagents to spread more uniformly ...
Low-Input CUT&RUN for Mouse Oocytes and Preimplantation …
WebSupply Solutions Roche - DIG Wash and Block Buffer Set 1 pc. Supplier: Supply Solutions SIGMA 11585762001 Roche - DIG Wash and Block Buffer Set 1 pc. Catalog No. 50-100-3351. $429.86 / Each of 1; Qty. Add to cart Description; SDS; Documents; Product Certifications; Promotions; Description. Description ... WebThe DIG Wash and Block Buffer Set is used at various steps of DIG hybridization and DIG detection. General description. Except for the Blocking solution, the 10x concentrated buffers are diluted to the desired concentrations with sterile double-distilled water (not included). Washing, blocking and detection buffers (10x concentrated) for the ... mercedes giveaway
Blocking Reagent Protocol & Troubleshooting - Sigma …
WebApr 29, 2024 · Two biological replicates of human K562 and H1 ES cells were washed twice with Wash Buffer, resuspended in Dig-wash buffer with 2 mM EDTA and arrayed in a … WebDIG Wash and Block Buffer Set* or † Washing buffer † Maleic acid buffer † Detection buffer 3.5 DNA transfer and fixation † UV- light box or † commercially available UV-cross linker CS × †2S or † 10 × SSC 3.6 Hybridization † Nylon membranes, positively charged* † Hybridization bags* or temperature resistant, sealable mercedes g fiyat listesi